2/15/2023 0 Comments Wilson and walker biochemistry pdfSystem identification techniques were used to model the cells' circularity in response to the harvesting solution and to quantify the time when the cells become circular and reach a plateau phase (inhibition time). The harvesting process was monitored under a microscope and image analysis was used to measure the cells' morphology (circularity) from the images. This study aimed at developing a non-invasive methodology to determine objectively and automatically the optimal time-point at which to inhibit the enzymatic reaction during cell harvesting. Determining the optimal time to inhibit the enzymatic reaction during cell harvesting is crucial to successfully detaching adherent cells from the substrate before irreversible cell damage is caused by prolonged cell exposure to the enzymes.
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